2024-2025 / SDOC0061-1

Molecular tools for bacterial system and ecology

Duration

15h Th

Number of credits

 Doctoral training in sciences (BMCB)3 crédits 

Lecturer

Annick Wilmotte

Language(s) of instruction

French language

Organisation and examination

All year long, with partial in January

Schedule

Schedule online

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

About 20 years ago, it became apparent that the study of the diversity of marine microorganisms cannot be conducted without a molecular approach, given that only a fraction of them can be isolated and cultured. In addition, as for the microorganisms that can be observed through a microscope, genetic diversity is larger than that which is determined on the basis of phenotypical characteristics (morphology....). The determination of diversity at the genetic level also allows us to understand ecological characteristics (geographical distribution, endemism, etc.).

Learning outcomes of the learning unit

To give to the students the knowledge that will allow them, on one hand, to understand and read in a critical manner the literature concerning the diversity of marine microorganisms, and on the other hand, to use molecular techniques in order to solve problems concerning the molecular diversity and ecology on which they may be working (master thesis, PhD, etc).

Prerequisite knowledge and skills

Having basic knowledge of genetic material: DNA, RNA, proteins. If this prerequisite is not met, part of the course may be devoted to a review.

Planned learning activities and teaching methods

Possibility of demonstrations in the laboratory

Mode of delivery (face to face, distance learning, hybrid learning)

Face-to-face teaching, in 1st or 2nd quadrisemester 1) Definitions 2) Why? 3) Molecular markers of diversity 4) Molecular techniques 5) Phylogenetic analyses and tree reconstructions 6) How  the genetic diversity is generated? 7) Examples of applications in molecular microbial ecology
8) If the student is interested, possibility of demonstrations in extraction of genomic DNA from cyanobacterial strains, PCR, electrophoresis, sequencing, phylogenetic analysis

Course materials and recommended or required readings

Copies of transparencies used in class

Exam(s) in session

Any session

- In-person

oral exam


Additional information:

2) Exam on the theory or presentation of a publication

Work placement(s)

Organisational remarks and main changes to the course

Optional course

Contacts

A. Wilmotte, InBios Chimie B6,
Phone : 04/366.33.87/38.56,
e-mail : awilmotte@ulg.ac.be

Association of one or more MOOCs