SBIM2034-1

Duration

12h Th, 10h Pr, 8h AUTR

Number of credits

	Master in biomedicine, research focus	3 crédits

Lecturer

François Boemer, Philippe Calmant, Benaïssa Elmoualij

Coordinator

Marie-Pierre Hayette

Language(s) of instruction

English language

Organisation and examination

Teaching in the second semester

Schedule

Schedule online

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

COORDINATOR : Hayette Marie-Pierre 25h Th, 25h Pr

Teachers : François Boemer, Philippe Calmant, Benaissa Elmoualij.

The module will include 4 modules provided by 3 groups of different teachers.

Quality assurance in laboratories - Philippe CALMANT - 2H Th

Content: Concept of quality assurance ; normative systems, accreditation bodies and audit procedures; documentation; quality manual and standardized procedures; quality coordinator's responsibility; validation of techniques and quality control; internal audits; corrective and preventive actions; complaint and claim management ; specific issues related to laboratories in clinical Biology.

Learning achievement: The course provides students with information about the implementation of a quality system in analysis laboratories, its management and development. It allows to get acquainted with the ISO 17025 standards, with a general scope for the test laboratories, and with the new ISO 15189 standards, applicable to biomedical laboratories.

Kind of teaching: face-to-face and distance learning - Written exam

Immunoassays - Benaïssa Elmoualij - 5H Th + 15H Pr

Learning achievement: The course firstly aims at introducing the immuno-detection tools. The basic tools will be antibodies. We will introduce the principles of the techniques used in immunological engineering. We will approach the reasons of the choice between various strategies used to produce antibodies and other tools as an alternative. The student will be led to understand the common applications of immunological engineering and to be able to propose the major elements of an experimental process that aims at solving a problem related to immuno-detection.

Kind of teaching: The exercises aim at illustrating the course by simulating problems related to immunological engineering met in research and development. Practical exercises will illustrate some key achievements in the field chosen in various scope of application: case of aptamers. During practical works, students will be made aware of the notion of the supports of immuno-detection and will carry out different experiments of immuno-detection by the development of ELISA and western blot.

GENERAL OVERVIEW AND STRUCTURE OF LESSONS

I- Introduction to immunological techniques
I-1. Introduction

I-2. Antibodies

I-3. Antigens / immunogens

I-4. Antigen-antibody reaction

II- Tools of immunoassays

II-1. Antibodies / antigens

II-2. Immunassays : « Use and limits »

II-3. Hybrid systems of immunoassays: aptamers, aptagenes, affinity peptides, etc.

II-4. Markers and performance measure of immunoassays
III- Scientific watch and development in techniques of immunoassays

Diagnosis of rare diseases: complementarity between metabolomics and genomics - François Boemer - 5H Th

Content: The course deals with the perspectives of genetic diagnosis of rare diseases thanks to metabolomics and genomics and their principles in diagnosis.

Kind of teaching: face-to-face, ex-cathedra classes, written exam

Kind of teaching :

Teaching is mainly based on face-to-face ex-cathedra classes during 5 hours, stimulating discussion during the lesson. The slideshows of theoretical classes are online and accessible via "my ULg."

Learning outcomes of the learning unit

Prerequisite knowledge and skills

Planned learning activities and teaching methods

Mode of delivery (face to face, distance learning, hybrid learning)

Course materials and recommended or required readings

Exam(s) in session

Any session

- In-person

written exam

Written work / report

Work placement(s)

Organisational remarks and main changes to the course

Contacts

Stéphanie Wauquier 04/323.22.90 (Stephanie.Wauquier@chuliege.be)

Advances II in Clinical Biology