Genetics

Duration

30h Th, 15h Pr

Number of credits

Lecturer

Claire Remacle

Language(s) of instruction

French language

Organisation and examination

Teaching in the first semester, review in January

Schedule

Schedule online

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

The theoretical course is divided into 10 chapters. The first two chapters are devoted to the organization of genes and genomes and to their replication and expression (transcription, translation). The three next chapters concern gene transmission, recombination and interaction between genes. Chapters 6 and 7 describe the types of mutations. Chapter 8 describes genetics of phages and bacteria.  Chapter 9 briefly describes the technology of recombinant DNA. Chapter 10 is devoted to organelle genetics.

Learning outcomes of the learning unit

Description of the basis of formal and molecular genetics.

Prerequisite knowledge and skills

Planned learning activities and teaching methods

Practical courses: one afternoon is devoted to bacterial conjugation in Escherichia coli (interrupted conjugation) and the other one to intragenic complementation of arg- markers in the unicellular green alga Chlamydomonas reinhardtii.


Bacterial conjugation: conjugation is made between a donor strain harboring a fertility plasmid and a recipient strain. Conjugation is interrupted at defined times. Transfer of plasmid is detected by plating bacteria on growth media supplemented with antibiotics.


Intragenic complementation: activity of the argininosuccinate lyase is measured before and after a heat shock in a wild-type haploid strain, in mutant haploid arg- strain and in a diploid strain showing intragenic complementation at the arg7 locus.


At the end of the theoretical course, one afternoon is devoted to the resolution of problems of genetics.

Mode of delivery (face to face, distance learning, hybrid learning)

Course materials and recommended or required readings

Syllabus and Power Point presentations on myULG.
Book: Introduction à l'analyse génétique, Griffith et al., DeBoeck

Exam(s) in session

Any session

- In-person

written exam


Further information:

Written exam (theory and problems)


 

Work placement(s)

Organisational remarks and main changes to the course

Contacts

Claire Remacle
Génétique et physiologie des microalgues, Institut de Botanique, Bât B22.
Tél 043663812, e-mail c.remacle@ulg.ac.be

Association of one or more MOOCs

Items online