Duration
15h Th
Number of credits
| Doctoral training in sciences (BMCB) | 3 crédits |
Lecturer
Moreno Galleni, André Matagne, N...
Coordinator
Language(s) of instruction
French language
Organisation and examination
Teaching in the second semester
Schedule
Units courses prerequisite and corequisite
Prerequisite or corequisite units are presented within each program
Learning unit contents
I. Strategy (part 1); II. Theory of chromatography; III. Extraction, precipitation, concentration, dialysis ; IV. Size exclusion chromatography; V. Hydrophobic Interaction Chromatography; VI. Ion exchange; VII. Affinity chromatography; VIII. Other chromatographic methods; IX. Analytical methods for protein analysis (incl. mass spectrometry); X. Strategy (part 2); XI. Scale-up
Learning outcomes of the learning unit
The aim of these lectures is to teach students fundamentals in protein purification methods.
Prerequisite knowledge and skills
The language used for the lectures will be english
Planned learning activities and teaching methods
Lectures, given with the help of powerpoint presentations, blackboard and white chalk. Furthermore, a laboratory visit will be organized to present AKTA systems.
Mode of delivery (face to face, distance learning, hybrid learning)
face-to-face
Course materials and recommended or required readings
Lecture notes will be available for free (on behalf of the graduate school SFMBBM°.
Refernce book:
Scopes, R.K., Protein purification. Principles and Practice, 3rd Edition, Springer-Verlag, 1994
Work placement(s)
Organisational remarks and main changes to the course
Students should register with Fabienne Julémont (fjulemont@ulg.ac.be) as soon as possible (program is generally available in May early).
Contacts
André Matagne or Moreno Galleni, Centre d'Ingénierie des Protéines, Département des Sciences de la Vie, Institut de Chimie B6, Allée de la Chimie, 3, Université de Liège, B4000 Liège (Sart-Tilman)