Techniques for cells and tissue cultures

Duration

8h Th, 20h Pr

Number of credits

Lecturer

Erik Maquoi

Language(s) of instruction

French language

Organisation and examination

Teaching in the first semester, review in January

Schedule

Schedule online

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

The course is subdivided in the following way: - cellular lines - techniques of aseptie - environment of the culture - primary cultures - techniques of observation in optical microscopy - contaminations - instability, variation and conservation - measurement of viability and cytotoxicity
- three-dimensional culture

Learning outcomes of the learning unit

The philosophy of the course of culture of cells is to communicate to the students the basic techniques used for the culture of animal or human cells in vitro

The objective continued are:
- maintenance of the viable isolated cells or the functional units apart from the organism;
- application of good laboratory practices;
- handling, which constitutes the best training for the student.

This course contributes to the learning outcomes V.2 of the MSc in biomedical engineering.

 

Prerequisite knowledge and skills

Good knowledge in cellular and tissue biology

Planned learning activities and teaching methods

- Preparation of sterilized material and media - Starting cells culture - Congelation -decongelation - Cells count - Passaging - Viability tests - Observations (phase contrast microscopy)
Local: salle de TP de Microbiologie

Mode of delivery (face to face, distance learning, hybrid learning)

First semester
Course: face-to-face/podcasts
TP: in L2 Laboratory

Recommended or required readings

Notes de travaux pratiques Dias (slideshow) on line
Ouvrage de référence: Culture of animal cells: a manual of basic technique and specialized applications. R. Ian Freshney - 7th edition - Wiley Editions (available at the library of Sciences de la Vie - CHU)

 
Theory: written examination in january

Work placement(s)

Organisational remarks and main changes to the course

Labcoat mandatory Laboratory book

Contacts

Dr. Erik Maquoi
Laboratoire de Biologie des Tumeurs et du Développement
Tour de Pathologie, B23 (+4), route 688
Avenue Hippocrate, 13 Sart - Tilman B 4000 Liège Tél: 04/366.25.31 E-mail: Erik.Maquoi@uliege.be

Association of one or more MOOCs

Items online