Duration
Theory : 10h Th
Specific practical work : 50h Pr
Number of credits
Lecturer
Theory : Laurence Delacroix, Rachelle Franzen, Carine Munaut, Agnès Noël, Christel Pequeux, Nor Eddine Sounni, Sabine Wislet
Specific practical work : Laurence Delacroix, Rachelle Franzen, Carine Munaut, Agnès Noël, Christel Pequeux, Nor Eddine Sounni, Sabine Wislet
Coordinator
Language(s) of instruction
French language
Organisation and examination
Teaching in the first semester, review in January
Schedule
Units courses prerequisite and corequisite
Prerequisite or corequisite units are presented within each program
Learning unit contents
LABO0001-2 (cycle Master passerelle SBIM)
This learning unit comprises theoretical classes (10h) and a majority of practical sessions (50h).
Theoretical lessons will provide :
- Basic information related to Good Laboratory Practice (security, how to write a Lab Book, ...)
- Information on DNA vectors and transgenic mice
- Basic concepts on immune reactions, required to understand IHC and WB techniques
The principles of each of these techniques will be extensively developed during the practical.
In addition, a session will be planned to prepare most of the required solutions and to revise appropriate calculation methods.
Before the practical sessions, an introduction will provide help on how to write and manage a Lab Book.
Theory
LABO0001-1 (cycle bachelier SBIM)
This learning unit comprises theoretical classes (10h) and a majority of practical sessions (100h).
Theoretical lessons will provide :
- Basic information related to Good Laboratory Practice (security, how to write a Lab Book, ...)
- Information on DNA vectors and transgenic mice
- Basic concepts on immune reactions, required to understand IHC and WB techniques
Practical sessions are divided into 5 themes :
Plasmid handling, DNA analysis (genotyping), RNA analysis (RT-PCR), IHC (Immunohistochemistry) and WB (Western Blot).
The principles of each of these techniques will be extensively developed during the practical.
In addition, a session will be planned to prepare most of the required solutions and to revise appropriate calculation methods.
Before the practical sessions, an introduction will provide help on how to write and manage a Lab Book.
LABO0001-2 (cycle Master passerelle SBIM)
This learning unit comprises theoretical classes (10h) and a majority of practical sessions (50h).
Theoretical lessons will provide :
- Basic information related to Good Laboratory Practice (security, how to write a Lab Book, ...)
- Information on DNA vectors and transgenic mice
- Basic concepts on immune reactions, required to understand IHC and WB techniques
The principles of each of these techniques will be extensively developed during the practical.
In addition, a session will be planned to prepare most of the required solutions and to revise appropriate calculation methods.
Before the practical sessions, an introduction will provide help on how to write and manage a Lab Book.
Specific practical work
LABO0001-1 (cycle bachelier SBIM)
This learning unit comprises theoretical classes (10h) and a majority of practical sessions (100h).
Theoretical lessons will provide :
- Basic information related to Good Laboratory Practice (security, how to write a Lab Book, ...)
- Information on DNA vectors and transgenic mice
- Basic concepts on immune reactions, required to understand IHC and WB techniques
Practical sessions are divided into 5 themes :
Plasmid handling, DNA analysis (genotyping), RNA analysis (RT-PCR), IHC (Immunohistochemistry) and WB (Western Blot).
The principles of each of these techniques will be extensively developed during the practical.
In addition, a session will be planned to prepare most of the required solutions and to revise appropriate calculation methods.
Before the practical sessions, an introduction will provide help on how to write and manage a Lab Book.
LABO0001-2 (cycle Master passerelle SBIM)
This learning unit comprises theoretical classes (10h) and a majority of practical sessions (50h).
Theoretical lessons will provide :
- Basic information related to Good Laboratory Practice (security, how to write a Lab Book, ...)
- Information on DNA vectors and transgenic mice
- Basic concepts on immune reactions, required to understand IHC and WB techniques
The principles of each of these techniques will be extensively developed during the practical.
In addition, a session will be planned to prepare most of the required solutions and to revise appropriate calculation methods.
Before the practical sessions, an introduction will provide help on how to write and manage a Lab Book.
Learning outcomes of the learning unit
- Conceptual and technical expertise of molecular biology and immunostaining assays, which are routinely performed in research labs.
- Acquiring practical expertise and autonomy in order to conduct future internships in academic laboratories or companies.
- Compliance to good laboratory practice and to the use of an official laboratory notebook to strictly record experimental design and data.
- Training in critical analysis of experimental data, in result synthesis and in oral presentation of the findings.
Theory
- Conceptual and technical expertise of molecular biology and immunostaining assays, which are routinely performed in research labs.
- Acquiring practical expertise and autonomy in order to conduct future internships in academic laboratories or companies.
- Compliance to good laboratory practice and to the use of an official laboratory notebook to strictly record experimental design and data.
- Training in critical analysis of experimental data, in result synthesis and in oral presentation of the findings.
Specific practical work
- Conceptual and technical expertise of molecular biology and immunostaining assays, which are routinely performed in research labs.
- Acquiring practical expertise and autonomy in order to conduct future internships in academic laboratories or companies.
- Compliance to good laboratory practice and to the use of an official laboratory notebook to strictly record experimental design and data.
- Training in critical analysis of experimental data, in result synthesis and in oral presentation of the findings.
Prerequisite knowledge and skills
Good skills in Biology, Molecular Biology and Protein Biochemistry is mandatory.
Theory
- BIOL0028-1: Biologie moléculaire
Specific practical work
- BIOL0028-1: Biologie moléculaire
Planned learning activities and teaching methods
Theoretical lessons are exclusively given ex-cathedra, on site.
Practical sessions in the laboratory are mandatory.
For each technique, students will conduct 2 rounds of experiments.
-"First Round": getting trained
Experiments are conducted with the help of lab assistants and the results are collected without being evaluated by the teaching staff. Students are invited to ask all their questions in order to get a deep understanding of the protocol and realize the goal and importance of each technical steps.
-"Second Round": Hands-on application
Experiments are conducted by students that are expected to work in autonomy. Nevertheless, lab assistants are there for supervision and will be ready to answer student's questions if they face any difficulty during the technical procedure. After this round, the data obtained will be recorded in the Lab Book and the quality of the results and their interpretation will be evaluated.
Every student will fill in his own lab Book.
Reports related to each session will also be included. Because each technique is unique, specific guidelines will be provided by the dedicated teacher after each session.
In case of orange sanitary code, presence on site will still be mandatory.
Theory
Theoretical lessons are exclusively given ex-cathedra, on site.
Practical sessions in the laboratory are mandatory.
For each technique, students will conduct 2 rounds of experiments.
-"First Round": getting trained
Experiments are conducted with the help of lab assistants and the results are collected without being evaluated by the teaching staff. Students are invited to ask all their questions in order to get a deep understanding of the protocol and realize the goal and importance of each technical steps.
-"Second Round": Hands-on application
Experiments are conducted by students that are expected to work in autonomy. Nevertheless, lab assistants are there for supervision and will be ready to answer student's questions if they face any difficulty during the technical procedure. After this round, the data obtained will be recorded in the Lab Book and the quality of the results and their interpretation will be evaluated.
Every student will fill in his own lab Book.
Reports related to each session will also be included. Because each technique is unique, specific guidelines will be provided by the dedicated teacher after each session.
In case of orange sanitary code, presence on site will still be mandatory.
Specific practical work
Theoretical lessons are exclusively given ex-cathedra, on site.
Practical sessions in the laboratory are mandatory.
For each technique, students will conduct 2 rounds of experiments.
-"First Round": getting trained
Experiments are conducted with the help of lab assistants and the results are collected without being evaluated by the teaching staff. Students are invited to ask all their questions in order to get a deep understanding of the protocol and realize the goal and importance of each technical steps.
-"Second Round": Hands-on application
Experiments are conducted by students that are expected to work in autonomy. Nevertheless, lab assistants are there for supervision and will be ready to answer student's questions if they face any difficulty during the technical procedure. After this round, the data obtained will be recorded in the Lab Book and the quality of the results and their interpretation will be evaluated.
Every student will fill in his own lab Book.
Reports related to each session will also be included. Because each technique is unique, specific guidelines will be provided by the dedicated teacher after each session.
In case of orange sanitary code, presence on site will still be mandatory.
Mode of delivery (face to face, distance learning, hybrid learning)
Face-to-face course
Additional information:
According to the sanitary rules, theoretical lessons will be on site (face-to-face) or remote (virtual classroom).
Practical sessions will be conducted by groups of 2 students. All the necessary documents and support are available on the e-campus page.
Theory
Face-to-face course
Additional information:
According to the sanitary rules, theoretical lessons will be on site (face-to-face) or remote (virtual classroom).
Practical sessions will be conducted by groups of 2 students. All the necessary documents and support are available on the e-campus page.
Specific practical work
Face-to-face course
Additional information:
According to the sanitary rules, theoretical lessons will be on site (face-to-face) or remote (virtual classroom).
Practical sessions will be conducted by groups of 2 students. All the necessary documents and support are available on the e-campus page.
Recommended or required readings
power point, documentations related to experiments including protocoles, video (eCampus).
Theory
power point, documentations related to experiments including protocoles, video (eCampus).
Specific practical work
power point, documentations related to experiments including protocoles, video (eCampus).
Assessment methods and criteria
Exam(s) in session
Any session
- In-person
oral exam
Written work / report
Continuous assessment
Out-of-session test(s)
Additional information:
- Assessment during the exam period (all exam periods) will comprise an oral evaluation on site (face-to-face).
- Personal work - after each 2nd round, the individual lab book and the specific report will be evaluated.
- Continuous assessment
- Evaluation out of the exam period
The global evaluation will take into account:
- an oral evaluation on site (face-to-face) or remote if applied by sanitary rules.
After an oral presentation of one of the techniques performed during the practical sessions, the student will be subjected to questions from the jury (60% of global evaluation). The grade obtained during this exam is absorptive. This means that any grade below 10/20 will absorb all other grades obtained (reports) and will lead to a second session for this oral exam.
- The quality of the reports provided after the 2nd round of experiments (quality of the experimental data, quality of the data analysis and interpretation in the Lab Book, specific exercises and questions in the report) will be evaluated. This accounts for 40% of the global evaluation.
- Personal preparation to the work, investment, autonomy and compliance to Good Laboratory Practise as well as to specific guidelines or rules from the teachers will be taken into account. In case of behavioral problems, shortcomings, lack of attendance or participation or non-compliance to the rules, a penalty (ranging from -1 to -3/20 on the global grade) may be applied.
- The capacity to perform dilution calculations. After the oral exam, a simple calculation related to the preparation of a chemical solution will be asked to students. If unsuccessful, the student will get a penalty of -1/20 on the global grade).
During the 2nd session of evaluation, the oral exam will be organized as for the 1st session. This grade will still account for 60% of the global evaluation (same calculation method and potential penalties as above). However, the grade will still be absorptive. Any grade below 10/20 will thus consist in the final grade for the course evaluation.
Theory
Exam(s) in session
Any session
- In-person
oral exam
Written work / report
Continuous assessment
Out-of-session test(s)
Additional information:
- Assessment during the exam period (all exam periods) will comprise an oral evaluation on site (face-to-face).
- Personal work - after each 2nd round, the individual lab book and the specific report will be evaluated.
- Continuous assessment
- Evaluation out of the exam period
The global evaluation will take into account:
- an oral evaluation on site (face-to-face) or remote if applied by sanitary rules.
After an oral presentation of one of the techniques performed during the practical sessions, the student will be subjected to questions from the jury (60% of global evaluation). The grade obtained during this exam is absorptive. This means that any grade below 10/20 will absorb all other grades obtained (reports) and will lead to a second session for this oral exam.
- The quality of the reports provided after the 2nd round of experiments (quality of the experimental data, quality of the data analysis and interpretation in the Lab Book, specific exercises and questions in the report) will be evaluated. This accounts for 40% of the global evaluation.
- Personal preparation to the work, investment, autonomy and compliance to Good Laboratory Practise as well as to specific guidelines or rules from the teachers will be taken into account. In case of behavioral problems, shortcomings, lack of attendance or participation or non-compliance to the rules, a penalty (ranging from -1 to -3/20 on the global grade) may be applied.
- The capacity to perform dilution calculations. After the oral exam, a simple calculation related to the preparation of a chemical solution will be asked to students. If unsuccessful, the student will get a penalty of -1/20 on the global grade).
During the 2nd session of evaluation, the oral exam will be organized as for the 1st session. This grade will still account for 60% of the global evaluation (same calculation method and potential penalties as above). However, the grade will still be absorptive. Any grade below 10/20 will thus consist in the final grade for the course evaluation.
Specific practical work
Exam(s) in session
Any session
- In-person
oral exam
Written work / report
Continuous assessment
Out-of-session test(s)
Additional information:
- Assessment during the exam period (all exam periods) will comprise an oral evaluation on site (face-to-face).
- Personal work - after each 2nd round, the individual lab book and the specific report will be evaluated.
- Continuous assessment
- Evaluation out of the exam period
The global evaluation will take into account:
- an oral evaluation on site (face-to-face) or remote if applied by sanitary rules.
After an oral presentation of one of the techniques performed during the practical sessions, the student will be subjected to questions from the jury (60% of global evaluation). The grade obtained during this exam is absorptive. This means that any grade below 10/20 will absorb all other grades obtained (reports) and will lead to a second session for this oral exam.
- The quality of the reports provided after the 2nd round of experiments (quality of the experimental data, quality of the data analysis and interpretation in the Lab Book, specific exercises and questions in the report) will be evaluated. This accounts for 40% of the global evaluation.
- Personal preparation to the work, investment, autonomy and compliance to Good Laboratory Practise as well as to specific guidelines or rules from the teachers will be taken into account. In case of behavioral problems, shortcomings, lack of attendance or participation or non-compliance to the rules, a penalty (ranging from -1 to -3/20 on the global grade) may be applied.
- The capacity to perform dilution calculations. After the oral exam, a simple calculation related to the preparation of a chemical solution will be asked to students. If unsuccessful, the student will get a penalty of -1/20 on the global grade).
During the 2nd session of evaluation, the oral exam will be organized as for the 1st session. This grade will still account for 60% of the global evaluation (same calculation method and potential penalties as above). However, the grade will still be absorptive. Any grade below 10/20 will thus consist in the final grade for the course evaluation.
Work placement(s)
Organizational remarks
- Before each practical, the student is expected to: read and understand the technical procedure, perform all dilution calculations required, visualize the video and the complementary information provided to fully understand the experiment and its principles.
- At the end of each practical, the student needs to show to a lab assistant that everything is clean and in order on his/her bench.
- The labcoat is provided by the student and wearing it inside the lab is mandatory.
- According to sanitary rules, face mask might be mandatory in the lab as well as during the theoretical lectures (2 masks per day).
Theory
- Before each practical, the student is expected to: read and understand the technical procedure, perform all dilution calculations required, visualize the video and the complementary information provided to fully understand the experiment and its principles.
- At the end of each practical, the student needs to show to a lab assistant that everything is clean and in order on his/her bench.
- The labcoat is provided by the student and wearing it inside the lab is mandatory.
- According to sanitary rules, face mask might be mandatory in the lab as well as during the theoretical lectures (2 masks per day).
Specific practical work
- Before each practical, the student is expected to: read and understand the technical procedure, perform all dilution calculations required, visualize the video and the complementary information provided to fully understand the experiment and its principles.
- At the end of each practical, the student needs to show to a lab assistant that everything is clean and in order on his/her bench.
- The labcoat is provided by the student and wearing it inside the lab is mandatory.
- According to sanitary rules, face mask might be mandatory in the lab as well as during the theoretical lectures (2 masks per day).
Contacts
Agnès Noel (theorical courses)
Tel 04/366 25 69
Email : agnes.noel@uliege.be
Christel Péqueux (coordinator, immunoréactions courses, TP dilutions, TP WB)
Tel 04/366 22 17
Email : c.pequeux@uliege.be
Laurence Delacroix (TP ARN)
Tel 04/366 21 78
Email : ldelacroix@uliege.be
Theory
Agnès Noel (theorical courses)
Tel 04/366 25 69
Email : agnes.noel@uliege.be
Christel Péqueux (coordinator, immunoréactions courses, TP dilutions, TP WB)
Tel 04/366 22 17
Email : c.pequeux@uliege.be
Laurence Delacroix (TP ARN)
Tel 04/366 21 78
Email : ldelacroix@uliege.be
Sabine Wislet (TP plasmide, electronic lab book)
Tel 04/366 39 88
Email : S.Wislet@uliege.be
Rachelle Franzen (TP IHC, introduction to IHC)
Tel 04/366 51 92
Email : rfranzen@uliege.be
Carine Munaut (TP ADN)
Tel 04/366 24 53
Email : c.munaut@uliege.be
Nor Eddine Sounni (TP ADN)
Tel 04/366 25 70
Email : nesounni@uliege.be
Specific practical work
Agnès Noel (theorical courses)
Tel 04/366 25 69
Email : agnes.noel@uliege.be
Christel Péqueux (coordinator, immunoréactions courses, TP dilutions, TP WB)
Tel 04/366 22 17
Email : c.pequeux@uliege.be
Laurence Delacroix (TP ARN)
Tel 04/366 21 78
Email : ldelacroix@uliege.be
Sabine Wislet (TP plasmide, electronic lab book)
Tel 04/366 39 88
Email : S.Wislet@uliege.be
Rachelle Franzen (TP IHC, introduction to IHC)
Tel 04/366 51 92
Email : rfranzen@uliege.be
Carine Munaut (TP ADN)
Tel 04/366 24 53
Email : c.munaut@uliege.be
Nor Eddine Sounni (TP ADN)
Tel 04/366 25 70
Email : nesounni@uliege.be