Duration
20h Th
Number of credits
| Master in bio-informatics and modelling (120 ECTS) | 3 crédits | |||
| Master in biochemistry and molecular and cell biology (120 ECTS) | 3 crédits |
Lecturer
Coordinator
Language(s) of instruction
French language
Organisation and examination
Teaching in the first semester, review in January
Schedule
Units courses prerequisite and corequisite
Prerequisite or corequisite units are presented within each program
Learning unit contents
Attention, suite à la réforme du master 2023_2024, ce cours n'est plus donné en présentiel et accessible uniquement aux étudiants répétants.
General introduction; energy in biochemical processes
Conversion of light energy to chemical energy:
- The first expriments of Emerson on photosynthesis: oxygen quantum yield and photosynthetic units
- The interaction of light with photosynthetic pigments; energy levels
- Deactivation processes of the excited state of chlorophyll; energy transfer and photochemistry
- Two photosystems: the 'red drop' effect: lateral distribution of photosystems in thylakoids
- Structure and function of photosynthetic reaction centers: general aspects; features of photosystem I and II reaction centers
- Organisation of photosystems and light-harvesting complexes
- Structure and function of cytochrome b6/f; analogy with mitochondrial complex III
- Linear and cyclic electron transport
- Secondary pathways of electron transport
- Carbon reduction and photorespiration
Oxidative phosphorylation and trans-membrane transport of metabolites:
- History and recalls: energetic catabolism
- The chemiosmotic theory: trans-membrane electrochemical gradient and photophosphorylation; the Mitchell hypothesis and basic experiments
- Mechanisms of electron transfers and of proton translocation in the respiratory chain
- Structure, composition, function and properties of respiratory complexes
- Respiratory control and energy dissipating systems in the mitochondria
- Structure, mechanism and function of ATP synthases
- Transfer of metabolites and ions through membranes: process diversity, structure and function of principal ionic exchangers
- Experimental methods to measure trans-membrane proton electrochemical gradients: lipophilic cations and electrochromic shift
Learning outcomes of the learning unit
Understanding of thermodynamial aspects of energetic metabolism at the organite and cell level
Acquisition of experimental and conceptual processes for scientific knowledge development in the fields of bioenergetics
Knowledge of energetic and mechanistic aspects of biological processes:
- of light excitation energy transformation
- of energetic coupling
- of conversion of redox energy into phosphate bound hydrolysis energy
- of trans-membrane metabolite transport
Capacity for solving problems that involve different forms of biochemical energy
Prerequisite knowledge and skills
cell metabolism; general notions of protein structure
Planned learning activities and teaching methods
Lectures; exercises
Mode of delivery (face to face, distance learning, hybrid learning)
Face-to-face
Recommended or required readings
Course notes or Power Point presentations
Any session :
- In-person
written exam ( multiple-choice questionnaire, open-ended questions )
- Remote
written exam ( multiple-choice questionnaire, open-ended questions )
- If evaluation in "hybrid"
preferred in-person
Additional information:
First session:
written exam including two multichoice tests (40 %) and three open-ended questions (60 %)
Second session:
written exam including two multichoice tests (40 %) and three open-ended questions (60 %)
Work placement(s)
Organisational remarks and main changes to the course
Contacts
Prof. Fabrice FRANCK: Laboratoire de Bioénergétique, B22, Tel: 043663904, email: F.Franck@ulg.ac.be
Dr. Pierre CARDOL: Laboratoire de Génétique et Physiologie des Microalgues, B22, Tel: 043663840, email: pierre.cardol@ulg.ac.be