2024-2025 / LABO0432-3

Techniques for cells and tissue cultures

Duration

8h Th, 20h Pr

Number of credits

 Master MSc. in Biomedical Engineering, professional focus2 crédits 
 Bachelor in biomedicine2 crédits 

Lecturer

Erik Maquoi, Marie-Julie Nokin

Language(s) of instruction

French language

Organisation and examination

Teaching in the second semester

Schedule

Schedule online

Units courses prerequisite and corequisite

Prerequisite or corequisite units are presented within each program

Learning unit contents

The course is subdivided in the following way:


- cellular lines
- techniques of aseptie
- environment of the culture
- primary cultures
- techniques of observation in optical microscopy
- contaminations
- instability, variation and conservation
- measurement of viability and cytotoxicity
- three-dimensional culture

Learning outcomes of the learning unit

The philosophy of the course of culture of cells is to communicate to the students the basic techniques used for the culture of animal or human cells in vitro

The objective continued are:
- maintenance of the viable isolated cells or the functional units apart from the organism;
- application of good laboratory practices;
- handling, which constitutes the best training for the student.

This course contributes to the learning outcomes V.2 of the MSc in biomedical engineering.

 

Prerequisite knowledge and skills

Good knowledge in cellular and tissue biology

Planned learning activities and teaching methods

- Preparation of sterilized material and media
- Starting cells culture
- Congelation -decongelation
- Cells count
- Cell passaging
- Viability tests
- Observations (phase contrast microscopy)

Local: salle de TP de Microbiologie

Mode of delivery (face to face, distance learning, hybrid learning)

Face-to-face course


Further information:

Second semester

Course: face-to-face

TP: in L2 Laboratory

Course materials and recommended or required readings

Platform(s) used for course materials:
- MyULiège


Further information:

Notes on practical work


Dias (slideshow) on line

A reference book for in-depth study: Culture of animal cells: a manual of basic technique and specialized applications. R. Ian Freshney - 7th edition - Wiley Editions (available at the library of Sciences de la Vie - CHU)

Exam(s) in session

Any session

- In-person

written exam ( open-ended questions )


Further information:

Theory: written examination in june

In-class assessment in the form of a written exam with open-ended questions

or

Distance assessment imposed by the health situation: distance written exam with open-ended questions

Work placement(s)

Organisational remarks and main changes to the course

Labcoat mandatory
TP notes (available online)

Contacts

Dr. Erik Maquoi
Laboratoire de Biologie des Tumeurs et du Développement
Tour de Pathologie, B23 (+4), route 688
Avenue Hippocrate, 13 Sart - Tilman B 4000 Liège Tél: 04/366.25.31 E-mail: Erik.Maquoi@uliege.be

Association of one or more MOOCs