Duration
8h Th, 20h Pr
Number of credits
Master MSc. in Biomedical Engineering, professional focus | 2 crédits | |||
Bachelor in biomedicine | 2 crédits |
Lecturer
Language(s) of instruction
French language
Organisation and examination
Teaching in the second semester
Schedule
Units courses prerequisite and corequisite
Prerequisite or corequisite units are presented within each program
Learning unit contents
The course is subdivided in the following way:
- cellular lines
- techniques of aseptie
- environment of the culture
- primary cultures
- techniques of observation in optical microscopy
- contaminations
- instability, variation and conservation
- measurement of viability and cytotoxicity
- three-dimensional culture
Learning outcomes of the learning unit
The philosophy of the course of culture of cells is to communicate to the students the basic techniques used for the culture of animal or human cells in vitro
The objective continued are:
- maintenance of the viable isolated cells or the functional units apart from the organism;
- application of good laboratory practices;
- handling, which constitutes the best training for the student.
This course contributes to the learning outcomes V.2 of the MSc in biomedical engineering.
Prerequisite knowledge and skills
Good knowledge in cellular and tissue biology
Planned learning activities and teaching methods
- Preparation of sterilized material and media
- Starting cells culture
- Congelation -decongelation
- Cells count
- Cell passaging
- Viability tests
- Observations (phase contrast microscopy)
Local: salle de TP de Microbiologie
Mode of delivery (face to face, distance learning, hybrid learning)
Face-to-face course
Further information:
Second semester
Course: face-to-face
TP: in L2 Laboratory
Course materials and recommended or required readings
Platform(s) used for course materials:
- MyULiège
Further information:
Notes on practical work
Dias (slideshow) on line
A reference book for in-depth study: Culture of animal cells: a manual of basic technique and specialized applications. R. Ian Freshney - 7th edition - Wiley Editions (available at the library of Sciences de la Vie - CHU)
Exam(s) in session
Any session
- In-person
written exam ( open-ended questions )
Further information:
Theory: written examination in june
In-class assessment in the form of a written exam with open-ended questions
or
Distance assessment imposed by the health situation: distance written exam with open-ended questions
Work placement(s)
Organisational remarks and main changes to the course
Labcoat mandatory
TP notes (available online)
Contacts
Dr. Erik Maquoi
Laboratoire de Biologie des Tumeurs et du Développement
Tour de Pathologie, B23 (+4), route 688
Avenue Hippocrate, 13
Sart - Tilman
B 4000 Liège
Tél: 04/366.25.31
E-mail: Erik.Maquoi@uliege.be