Duration
20h Th, 10h Mon. WS
Number of credits
| Master in bio-informatics and modelling (120 ECTS) | 3 crédits | |||
| Master in biochemistry and molecular and cell biology (120 ECTS) | 3 crédits |
Lecturer
Language(s) of instruction
French language
Organisation and examination
Teaching in the first semester, review in January
Schedule
Units courses prerequisite and corequisite
Prerequisite or corequisite units are presented within each program
Learning unit contents
This course is organized as follows: Chapter 1: General properties of enzymes. Chapter 2: Steady-state kinetics. Chapter 3: Enzyme inhibition. Chapter 4: Effect of pH, temperature, viscosity and other experimental factors. Chapter 5: Reactions of two substrates. Chapter 6: Transient-state kinetics. Chapter 7: Cooperativity and allosteric interactions. Chapter 8: Enzyme inactivators. Chapter 9: Enzyme cofactors. Chapter 10: Mechanism of chymotrypsin. Chapter 11: Basic catalytic principles. Chapter 12: Regulation of catalytic activity.
In addition, a few sessions will be dedicated to specific topics (e.g. nanobodies as enzyme inhibitors, enzymes of the peptidoglycan synthesis pathway, the formation of disulfide bridges in proteins), taught by specialists. These sessions aim to illustrate the great diversity of biolochemical reactions catalyzed by enzymes.
Learning outcomes of the learning unit
The aim of the course is to make students familiar with enzyme mechanisms and fundamentals of enzyme kinetics.
Prerequisite knowledge and skills
Course on "Equilibriums in biochemistry and enzyme kinetics" (BAC Biology), which includes an introduction to enzyme catalysis (chapters 1-3; note that both theoretical and practical aspects are covered).
Planned learning activities and teaching methods
Most lectures are given using the blackboard and white chalk. Problems tutorials are organized in relation with some chapters.
Mode of delivery (face to face, distance learning, hybrid learning)
Face-to-face course
Additional information:
Due to the 2023-2024 reform of the BBMC Master, this course is no longer organised and is only accessible to students who did not pass the exam before the reform started.
Recommended or required readings
Lecture notes will be available as the course progresses.
Reference book:
A. Cornish-Bowden, Fundamentals of enzyme kinetics, fourth edition, Wiley-Blackwell, 2012.
A.R. Fersht: Structure and mechanism in protein science, W.H. Freeman and Co, 1999.
Exam(s) in session
Any session
- In-person
written exam ( open-ended questions )
Additional information:
Written exam
Work placement(s)
Non applicable
Organisational remarks and main changes to the course
Lectures will be organized according to the schedule known by the students. The professor will inform the students in advance of any possible modification to this
Contacts
André Matagne, PhD, Full Professor, Enzymology and Protein Folding, Centre for Protein Engineering, Life Science Department, Institut de Chimie B6c (room 3/61), Quartier Agora, Allée du 6 Août, 13, University of Liège, B4000 Liège (Sart-Tilman), Tel.: +32 (0)4 3663419, Email: amatagne@ulg.ac.be